Operation of the Holographic bio Information-by the Laser with the MBER effect

Operation of the Holographic bio Information-by the Laser with the MBER effect

G.G. Tertyshny, P.P. Garyaev
English version, prepared for online publication

Introduction: On the Foundations of the Method

For understanding the operating principles of the holographic information-laser transformer used by us — in fact, a quantum biocomputer [26] — the most useful works proved to be those of the classic of dynamic holography, Yu.N. Denisyuk [17]. He developed the foundations of holographic imaging of material structures, including dynamic ones moving in space-time (for example, Doppler holography). This is especially important for our theoretical constructions and their embodiment in concrete devices, since organisms are, from the holographic standpoint, non-stationary media. Using Denisyuk’s principles as the theoretical base, we succeeded in experimentally proving their applicability to the functioning of biosystems. This gave an impetus for further development of the theory of control over biological and physical objects by means of spatial-holographic translation of modulatory information, carried out in several ways in biological and physical objects [2–13, 21]. The essence of this phenomenon is based on the hypothesis of the unity of wave and material processes occurring in all closed and open cyclic systems [18]. The translation of modulatory information from a donor object to a recipient object proceeds by means of rectilinearly propagating, mutually penetrating waves carrying multi-level modulatory information.

One of the theoretical justifications of the method of holographic bio-control can be the physical-mathematical model we used to develop a method of forming an incoherent polarization-dynamic biological hologram by means of the optical properties of cell nuclei (chromosomes) acting as spherical lenses (quasi-lenses), with polarization-optical components in the form of DNA cholesteric liquid crystals.

Let us consider the formalized description of this process, which was proposed for recording color holograms without the use of lasers [22]. We note in passing that chromosomes cannot literally be regarded as lasers. What relates them to lasers is only that they are sources of coherent radiation. Adapting the formalism of [22] to a biological system, we describe the intracellular processes taking place. Following this, we give a mathematical justification of the operability of the incoherent polarization-holographic amplitude-phase quasi-lens and, by this path, arrive at an explanation of the essence of the method of wave control over organisms located in the “far zone.” In a certain sense, a biosystem is a complex associate of optically active substances, polarizers rotating the plane of polarization of optical radiation passing through them — and this is well known [19, 27]. However, the principles of bioholographic control using light polarization were previously considered only by us.

Theoretical Model of the Quasi-Lens

For the justification of the method of obtaining an incoherent polarization-dynamic hologram (including a biohologram) formed with the help of a quasi-lens, we present from the physical-mathematical model [22] the final formula for the distribution of light intensity in the hologram registration plane:

(1) I(r) = I₀ · K · [1 + cos φ(r)], where the polarization-modulated interference term encodes the donor structure in the intensity distribution of the registration plane.

For comparison, we show the distribution of intensity in an ordinary coherent axial hologram of a donor point, obtained as a result of interference of the object spherical wave and the reference plane wave:

(3) I(r) = I₀ · [1 + cos(2πr² / (λz))],

where:

• I₀ — the intensity of light scattered by a certain point located on the probed biological donor object;
• K — the transfer coefficient for intensity;
• φ — the angle of rotation of the polarization of light upon passage of a normally incident ray through some optically active component of the organism. As such a component, for work, for example, in the “near zone,” practically any metabolite or subcellular structure may act, including DNA [27];
• z — the distance from the point of the biological donor object to the hologram registration plane of the recipient, and r — the distance from the axial line passing through the center of the hologram recorder to the point at which the ray emanating from the point of the biological donor object falls;
• λ — the average wavelength of the translating signal emanating from the light source, which in this case will be the cell nucleus or some aggregate of them [28]. For modeling this process in vitro we use a special laser [24, 29].

From formula (3) it is seen that inside a circle of radius r_N there will be located N bright (or dark) rings, determined by the formula:

(4) r_N = √(Nλz).

In this case the impulse response h (the holographic point-spread function) has the form:

(5) h(r) ∝ exp(iπr² / λz),

and the holographic transfer function can be determined from the Fourier transform of expression (5). The obtained hologram contains complete volumetric information about the spatial characteristics of the holographed object, or about the spatial distribution of the points of the donor surface relative to the hologram registration plane of the recipient.

Thus, the solution of our problem is analogous to the traditional one. At the same time, it is evident that the method described above differs fundamentally from known interference methods and provides incontestable advantages.

Advantages of the Method

First, along with the laser monochromaticity and coherence of the light of cell nuclei — both in the situation of endogenous biowave processes and under artificial translation of the signal — there is used the dispersion rotation capability of the optically active medium of the organism and the spatially locally distributed polarization filtration through a quasi-lens for work in the “far zone.” This is quite sufficient for the recipient to perceive the wave biosignal-image of the donor without distortions under conditions of the donor’s dynamics as a non-stationary medium. The fundamental property of cellular structures of biosystems to be optically active — that is, to polarize light — probably allows organisms to use even non-coherent light for vibration-stable registration and reconstruction of their own holograms even without laser sources of light. This happens when biosystems, for example plants, use natural solar illumination over the whole spectrum from UV to IR range for biomophogenesis. Vibration stability is determined by the magnitude of the polarization-optical rotation capability and, consequently, by the thickness of the layer of optically active medium of cell nuclei for work in the “near zone” and by the thickness of the layer of optically active medium of the quasi-lens for work in the “far zone.” It is known that the rotation capability of some liquid crystals reaches 40,000 degrees/mm, which, when used in the holographic information-laser transformer — the main component of the quantum biocomputer — is quite sufficient for wide use of this method along the line of polarization-holographic translation of genetic-metabolic information and holographic control of biosystems.

Taking into account the proposed mathematical model, we justified the above-mentioned model of the liquid-crystal cell nucleus (or continuum of nuclei) as a biological quasi-lens. It allowed us to create the first bioholographic installation — in fact a quantum-analog biocomputer — which performs the following real functions of wave control over the recipient biosystem:

1. Reading from the biosystem/biostructure that is the donor of the wave equivalent of genetic-metabolic information and/or trigger wave signals, which switch on the corresponding programs in the recipient biosystem.
2. Transfer, with the help of a specially designed and manufactured quasi-lens, of polarization-holographic dynamic modulatory information from the donor to the recipient located in the “far zone.”
3. Addressed (targeted) introduction of this information into the recipient biosystem.
4. Strategic control of the metabolism of the recipient biosystem.

These four functions we demonstrated in Russia (Moscow) in 2000, and then in Canada (Toronto) in 2002. We repeated this work in Russia in an extended variant (Nizhny Novgorod) in 2007 [24]. Subsequently we discovered other biological phenomena (see below) connected with the application of technologies of this kind [29]. This research direction, born at the Institute of Control Sciences of the Russian Academy of Sciences (IPU RAN), is not limited to the practical use of only the first model of the quantum biocomputer. On the basis of the theory given by us earlier [3, 4, 7–13, 25, 29] and developed in the present work — thanks mainly to the efforts of G.G. Tertyshny — it may be expected that an extensive family of quantum biocomputers will be created, using the whole range of coherent probing polarized radiations from UV to IR ranges.

Experimental Confirmations of the Proposed Theory: The Toronto Experiments of 2002 Compared with the Nizhny Novgorod Work of 2007

Toronto Experiments (full description of methods in [24, 29])

The graphs present four series of experiments: four groups of rats with diabetes (pancreas (PZh) destructured by injection of alloxan) were irradiated with MBER — a modulated broadband electromagnetic radiation field obtained by means of a Helium-Neon (HeNe) laser, 632.8 nm (red), operating with the MBER effect — carrying information read from fresh preparations of pancreas and spleen of a newborn rat of the same line. On the Y axis — blood sugar level; on the X axis — days from the start of the experiment. Arrow at day 1 — injection of alloxan (200 mg/kg); second arrow — MBER irradiation. From top to bottom (experiment): 1st group — irradiation at a distance of 1 cm by laser beam and MBER. 2nd group — MBER irradiation at a distance of about 3 m. 3rd group — MBER irradiation at a distance of about 15 km. 4th group — MBER irradiation at a distance of about 15 km. It can be seen that on days 9–12 the blood sugar level of the animals practically returns to normal. All 4 groups survived. In the control (60 rats), where MBER irradiation was absent, 95% of the animals died on days 4–7. The results were subjected to statistical analysis by Student’s criterion; the difference in blood sugar level indicators within groups is significant: p < 0.001.

We supposed that the normalization of blood sugar level in the experiment testified to regeneration of the pancreas. In order to finally verify this, several series of extended experiments were set up [24, 29].

Experiments of [24]

Dynamics of change of blood glucose level and course of alloxan diabetes in rats. The animals were given alloxan at a dose of 200 mg/kg and exposed to MBER — the modulated broadband electromagnetic radiation field generated using a Helium-Neon (HeNe) laser, 632.8 nm (red), with the MBER effect — from the tissue of the pancreas and spleen of a newborn rat. The exposure was carried out over 4 days with 30-minute exposures. The distance from the radiation source to the animals was 70 cm. The MBER exposure regimen: 10 minutes using pancreas tissue, 10 minutes using spleen tissue, and 10 minutes using pancreas tissue. Day zero corresponds to the day of alloxan injection. The MBER exposure days were days 3, 4, 5 and 6 after alloxan injection. This graph was not included in publication [24] due to volume limits, but the result was used for the conclusions. The statistics are analogous to the Toronto experiments. It can be seen that the result is identical to the Toronto experiments.

Influence of MBER on animal lethality (%) upon injection of alloxan (200 mg/kg for group 2 rats and 300 mg/kg for group 3; 10 rats per group) for modeling diabetes. Control — animals without any experimental exposures. The difference in mortality indicators between groups is significant by Student’s criterion (p < 0.001). It can be seen that mortality in the control group rises sharply and by day 3 amounts to 70%. On day 3, groups 2 and 3 were irradiated with MBER — the field produced by probing fresh preparations of pancreas and spleen of newborn rats of the same line with a Helium-Neon (HeNe) laser, 632.8 nm (red), with the MBER effect. Mortality for group 3 was zero on days 3–5; only on days 6–7 did it amount to 20%, and 30% by day 40. For group 2 a sharp difference from the control is also observed, with weak growth of mortality on days 7–40. The difference in mortality rates between groups 2 and 3 is explained by the fact that the distance between the MBER source and the rats of group 3 was 70 cm, while between the MBER source and the rats of group 2 it was about 20 m. In addition, group 2 was shielded from the MBER source by a concrete ceiling and walls.

Experiments of [29]

The essence of the experiments was that the animals were first irradiated with MBER and only then given alloxan, in order to check the effect of preliminary irradiation of the animals on the development of alloxan diabetes. Four groups of 20 animals each were used. Group 1 — control (no external artificial exposure). Group 2 animals at the moment of exposure were placed at a distance of 20 m from the MBER source in a room shielded with concrete. Alloxan diabetes in this group was induced one month after the last MBER exposure. Groups 3 and 4 were placed at a distance of 70 cm from the MBER source. Alloxan diabetes in these groups was induced one day after the last MBER exposure. Group 4 (placebo) was exposed to MBER with probing of empty glasses (in the experiments, fresh preparations of pancreas and spleen of newborn rats of the same line were placed between the glasses; in the placebo variant the glasses were empty). Day zero — the day of alloxan injection. Statistical processing of results was carried out using the software packages “Statistica 6.0” and “MS Excel for Windows.”

In all experimental series, the MBER field was obtained by probing the donor preparations with a Helium-Neon (HeNe) laser, 632.8 nm (red), operating with the MBER effect: the polarization-holographic dynamic modulation of the HeNe beam by the donor tissue was converted by the built-in quadratic photodetector into a broadband electromagnetic signal (0.5–1.5 MHz band), which constituted the MBER field acting on the animals.

Table. Blood glucose level in animals after alloxan injection at a dose of 200 mg/kg body weight (mmol/L)

Group	Day zero	Day 2	Day 3	Day 4
Group 1 (control), n=20	5.97 ± 1.38 (n=20)	25.93 ± 8.16** (n=18)	24.65 ± 9.78** (n=8)	22.63 ± 10.7** (n=6)
Group 2 (experiment), n=20	6.78 ± 0.83 (n=20)	24.7 ± 9.17*** (n=20)	18.99 ± 8.0*** (n=18)	21.93 ± 9.91*** (n=18)
Group 3 (experiment), n=20	5.23 ± 0.69 (n=20)	8.00 ± 6.32* (n=20)	6.44 ± 4.39* (n=20)	4.88 ± 2.90* (n=20)
Group 4 (placebo), n=10	4.9 ± 0.85 (n=10)	26.97 ± 6.85**** (n=8)	— ^ (n=1)	— ^ (n=1)

* — blood glucose level of group 3 animals on days 2, 3 and 4 of alloxan diabetes modeling differs significantly (p < 0.05) from that of groups 1 and 2 on days 2, 3 and 4, and also differs significantly (p < 0.05) from that of group 4 animals on day 2; ** — blood glucose level of group 1 animals on days 2, 3 and 4 differs significantly (p < 0.05) from the initial value; *** — blood glucose level of group 2 animals on days 2, 3 and 4 differs significantly (p < 0.05) from the initial value; **** — blood glucose level of group 4 animals on day 2 differs significantly (p < 0.05) from the initial value; ^ — in group 4 on days 3 and 4 only one surviving animal was observed.

Animal survival (%) in the studied groups during modeling of alloxan diabetes (another presentation of the results given in the Table).

Influence of preventive MBER exposure on the course of alloxan diabetes in rats of group 3

The animals were given alloxan at a dose of 200 mg/kg one day after preventive MBER exposure. The exposure was carried out over 4 days, 30 minutes daily. Distance from the radiation source: 70 cm. The MBER exposure regimen: 10 minutes using pancreas tissue, 10 minutes using spleen tissue, and 10 minutes using pancreas tissue. Here again, the MBER field was obtained by means of a Helium-Neon (HeNe) laser, 632.8 nm (red), operating with the MBER effect probing the donor preparations. Day zero corresponds to the day of alloxan injection. It can be seen that alloxan does not exert a pathological effect on sugar metabolism in the animals.

Histology: Structure of pancreatic tissue, islets of Langerhans

• a — intact rats;
• b — group 1 (control), after alloxan injection at 200 mg/kg;
• c — group 2 on day 8 after alloxan injection at 200 mg/kg. One month before modeling, the animals of this group were subjected to preliminary MBER exposure and were at a distance of 20 m from the source in the basement room of the laboratory;
• d — group 2, 1.5 months after alloxan injection at 200 mg/kg (same pre-exposure conditions as in c);
• e — group 3 on day 8 after alloxan injection at 200 mg/kg. One day before modeling, the animals of this group underwent preliminary MBER exposure at a distance of 70 cm from the source;
• f — group 3, 1.5 months after alloxan injection at 200 mg/kg (animals underwent preliminary MBER exposure at 70 cm).

Magnification ×400 and ×100; hematoxylin and eosin staining. The Table, graphs, and histological analysis clearly show that preventive MBER exposure — in contrast to control and placebo — exerts a protective effect against the action of alloxan. This may mean that a phenomenon of wave immunity has been discovered, which substantially complements the information about the ability of MBER to induce regeneration of the pancreas in situ.

How the MBER Field Is Produced: Summary

For clarity, we summarize the generation chain of the MBER field used in all experiments described above:

1. A Helium-Neon (HeNe) laser, 632.8 nm (red), operating with the MBER effect, emits orthogonally polarized modes that probe the semi-transparent donor preparation (fresh pancreas/spleen tissue of newborn rats of the same line).
2. The donor tissue acts as a polarization-holographic modulator; polarization quasi-rings of Newton encoding its dynamic genetic-metabolic status are formed.
3. A quadratic photodetector built into the laser tube converts this polarization-dynamic modulation into an alternating electromagnetic signal in the 0.5–1.5 MHz band.
4. The resulting modulated broadband electromagnetic radiation — MBER — is delivered to the recipient animals either directly (near zone, 1 cm–70 cm), through obstacles (concrete shielding, ~20 m), or translated to the far zone (up to ~15 km in the Toronto experiments).

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